My first real money hand of any consequence- advice?

[flyangler]

My first real money experience online (I've played live home games many times), and I got served. This is about 12 hands in to a .25/.50 ring game. Any advice?

Seat 1: DB_88 ($4.55 in chips)
Seat 2: NutzoJoe ($39.25 in chips)
Seat 3: davidkurosh ($7.85 in chips)
Seat 4: jaymes-y ($12 in chips)
Seat 5: Mike Oxmall2 ($21.60 in chips)
Seat 6: NewKnee9 ($15.50 in chips)
Seat 7: waveridin59 ($15.15 in chips)
Seat 8: alfy007 ($16.55 in chips)
Seat 9: mulchachie ($11.30 in chips)
Seat 10: flyangler [QS,10D] ($4.25 in chips)

ANTES/BLINDS
waveridin59 posts blind ($0.10), alfy007 posts blind ($0.25).

PRE-FLOP
mulchachie calls $0.25, flyangler calls $0.25, DB_88 folds, NutzoJoe calls $0.25, davidkurosh folds, jaymes-y calls $0.25, Mike Oxmall2 calls $0.25, NewKnee9 folds, waveridin59 folds, alfy007 checks.

FLOP [board cards 5C,10C,6D ]
alfy007 checks, mulchachie bets $0.25, flyangler calls $0.25, NutzoJoe folds, jaymes-y calls $0.25, Mike Oxmall2 folds, alfy007 calls $0.25.

TURN [board cards 5C,10C,6D,10H ]
alfy007 checks, mulchachie bets $0.50, flyangler bets $1, jaymes-y folds, alfy007 folds, mulchachie bets $1,flyangler bets $1, mulchachie calls $0.50.

RIVER [board cards 5C,10C,6D,10H,4H ]
mulchachie bets $0.50, flyangler bets $1, mulchachie calls $0.50.

SHOWDOWN
flyangler shows [ QS,10D ]
mulchachie shows [ 10S,KS ]
mulchachie wins $8.20.




SUMMARY
Dealer: NewKnee9
Pot: $8.60, (including rake: $0.40)
DB_88, loses $0
NutzoJoe, loses $0.25
davidkurosh, loses $0
jaymes-y, loses $0.50
Mike Oxmall2, loses $0.25
NewKnee9, loses $0
waveridin59, loses $0.10
alfy007, loses $0.50
mulchachie, bets $3.50, collects $8.20, net $4.70
flyangler, loses $3.50

What should I have done differently? Is the reraise a redflag that I should have seen as a real threat and just called (I thought maybe she had two pair)? Any advice would be great.