Having won 40 x the buy in , its time to move up. Give yourself 2-4 weeks to adjust to the different subtleties of the game there. Losing 4-8 buyins during that time should be viewed as tuition. Any more than that and maybe step down for a while until you build your stack back up to $1000.
Play weak, tight to start, until you have seen the plays made there, that you seldom or never see at $25nl.
ie: you make pot size bet on flop $5 , and get reraised for $10 more. this is hard to call, but they often have 4 to a flush, not trips.
-some guy with A4 hits his 4, and hangs in to the hand to the river where he hits his A. I found this often. They call this a tough player. they are rare at $25nl, but they abound at $100, and there are many at $50.
-back door flush draws, followed by an all in bet can be very expensive here.
IMO, you must immediately defend your hand "vigorously" here, of you'll be overtaken. This applies particularly to straights.