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The players at these two levels are incredibly weak. You have to adjust your play to it. Bluffing them is not usually the best idea. It's better to wait for real hands and extract value from them and pick up small pots with infrequent bluffs.

If you're losing at this game you're probably making too many mistakes for one. And two... variance will definately come in to play.

Be careful what you put your money in with, and learn about things like pot control and the volatile irrational state of your opponents.

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I agree that you certainly have to adjust your game. 25NL and 50NL are mostly filled with the same bad players and does not play very differently. Bluffing at the tables depends on who your playing. Generally, restrain from bluffing too often, but you will ocassionally come across a table that you can just run over, although that is usually the exception, not the rule.