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Or you could just have a worn fret there -

shouldn't cost much to fix though - at best you're talking about replacing what, one fret?

RB

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This is what has me nervous. The frets look fine.

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The fret may be from a defective lot in terms of the actual height of the fret. It may also have to do with the fret slot on the fretboard or neck being mis-machined. There also could have been a little bit of debris in the area where the fret is installed. A little chunk of saw dust in the area could raise the fret, let's say .015" - .030" higher than the rest putting one area out of tune.

Just a couple ideas especially if the fret appears visually ok. Take it to a professional.

Rock On