[C-Dog]

Boot to a floppy drive with NTFS support, and delete the file from a command prompt?

Or, you could add a command to delete the file at the beginning of your startup routine, and see if that works. You will probably need to boot to a prompt of some type though.

Try Knoppix, you can burn the CD and launch a fully functional linux OS from it, you should be able to delete the file in that.

C-Dog