Hand 1, I agree w/ the previous posts. That turn bet was pretty much a donation, as he wasn't buying your bluff and you're almost certainly behind. No reason to pour more money into the hand.

Hand 2. It's hard to make the call w/out a read of the player. His bet on the river represents a boat. But, does he have it, or is he the type of player who would bet 3 queens or Queens up into a possible flush board? Conversely, is he the type of player who would make a delayed bet on a flush of his own, in the face of that scary queen?

Against a LAG, I call; Tight, passive, fold

It's like Phil Ivey says "It all comes down to whether your opponent has the hand he's representing."